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Figure 1 | BMC Genomics

Figure 1

From: In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column

Figure 1

Targeting constructs for Bapx1 . (A) Wildtype Bapx1 allele and targeted Bapx1 alleles producing EGFP via IRES in the wildtype condition (Bapx1tm2.Tlu) and via the endogenous Bapx1 promoter in the loss-of function condition (Bapx1tm4.Tlu) as adapted from [17]. Southern blot with internal and external probes to verify the targeting event of the Bapx1tm2.Tlu(B) and Bapx1tm4.Tlu(C) alleles. Routine PCR genotyping to verify the wildtype and targeted allele (D) and the presence and absence of the neomycin cassette (E).

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