Expression profiling of E12.5 Bapx1 and Sox9 producing cells from embryos. (A) Endogenous EGFP tagged E12.5 day whole embryo showing EGFP in domains of expression of Bapx1 and the five distinct organs of expression- spleen, gut, vertebral column (VC), forelimb (FL) and hindlimb (HL). (B) Gene expression profiling of cells sorted from five organs in the wildtype embryo as compared to null showing Bapx1 can function as either an activator or repressor in these organs. (C) Venn diagram showing almost non-overlapping regulation in Bapx1 expressing organs. GO annotations of genes regulated by Bapx1 in vertebral column (D), gut (E) and spleen (F) where the x-axis is -log10 (p-value). (G) E12.5 day whole embryo showing EGFP expression under the endogenous Sox9 promoter in expected domains. (H) GO annotations of genes regulated by Sox9 in the vertebral column (VC) where the x-axis is -log10 (p-value).