Validation of Bapx1 target genes by RNA in situ hybridization. RNA in situ hybridization for Bapx1 regulated genes in E12.5 wildtype and Bapx1 null embryos validating the expression profiling data for Bapx1 (A and B), Chad (C and D), Ctfg (E and F) , Uncx (G and H), Wwp2 (I and J) in the vertebral column. The arrow marks the loss of expression of the genes in the rostral part of the vertebral column. Similar loss of expression is seen in the spleen of the null embryo for Bapx1 (K and L), Akap12 (M and N) and Gyg (O and P). Abbreviations: Sp, Spleen; St, Stomach; P, Pancreas.