Figure 1

Multiplex PCR based target enrichment. A typical design used for targeted amplicon sequencing. The target is tiled with overlapping amplicons. Each amplicon is represented by a forward primer (F#) and a reverse primer (R#). Any two amplicons that overlap with each other are added to separate multiplex PCR pools to avoid undesired PCR products. The design above requires three multiplex PCR pools denoted by P1, P2 and P3.