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Table 1 Sensitivity of calling simulated variants for different values of u

From: Edge effects in calling variants from targeted amplicon sequencing

u(minimum distance of the Uracil base from 3’ end of primer) Median value of d(actual distance of the Uracil base from the 3’ end of the primer) No. of simulated variants Recovered variants % of variants recovered No. of false-negatives
1 2 15,871 13,041 82.17 2,830
2 3 15,871 15,474 97.50 397
3 5 15,871 15,834 99.77 37
4 6 15,871 15,858 99.92 13
5 7 15,871 15,866 99.97 5
6 8 15,871 15,869 99.99 2
7 9 15,871 15,870 99.99 1
8 10 15,871 15,871 100.00 0