Figure 5

SRAMs induced with 5AZA are strongly associated with M cell lines. A) GSEA of SRAMs in A549 (M-type cell) finds a substantial proportion of genes to have a negative association with gene sets that are repressed during cell migration and EMT states; this relationship is readily reversed by 5AZA treatment. B) Data from Shames et al. [31] demonstrates more M-SRAMs being induced (≥ 2-fold more) than suppressed in M cells, which is not apparent in the E cells. A similar trend is noticed in the E cells for the E-SRAMs, although not significant. C) Bayesian statistical method to determine the posterior probability that more genes increase (rather than decrease) expression after treatment with 5AZA by the type of cell line, using either an uninformative prior (pi0) or a conservative prior (pi1).