Table 3 BCL11A and HBS1L-MYB minisequencing primers
SNP | Primer direction | Minisequencing primers (5′-3′)a | Size (bp) | Concentration (μM) | Detected allelesb |
|---|---|---|---|---|---|
BCL11A | Â | Â | Â | Â | Â |
rs11886868 | F | CAGAATCATTCTGCTCTGTG | 20 | 0.02 | G/A |
rs4671393 | F | acaaGGAATCTTAATTTCCTGCAC | 24 | 0.1 | T/C |
rs7557939 | F | aaCACCCTCTCTCACTCTTG | 20 | 0.1 | C/T |
rs6732518 | F | aactaggtgccacgtcgtgaaagtctgacaaGACTCCTGAGAGCATGT | 48 | 0.02 | G/A |
rs10189857 | F | gtctgacaaGCTTGTCACAGTTCTCTAC | 28 | 0.2 | T/C |
rs6545816 | F | tctgacaaATTAAAATTAAGCCTCTTGCTTTT | 32 | 0.05 | T/G |
rs7599488 | R | aactgactaaactaggtgccacgtcgtgaaagtctgacaaTTAGTCTCAGCCACCTG | 57 | 0.2 | G/A |
rs1427407 | F | gtctgacaaTTCAAGTAGATATCAGAAGGGAA | 32 | 0.1 | A/C |
rs766432 | F | aggtgccacgtcgtgaaagtctgacaaATGAATGACTTTTGTTGTATGTAAA | 52 | 0.05 | G/T |
rs10184550 | F | ctgacaaTTGCTTTGATAAGTATCTATACAAATATT | 36 | 0.05 | C/T |
rs7606173 | F | taggtgccacgtcgtgaaagtctgacaaTGTCCTGTGAGCGGTC | 44 | 0.2 | C/G |
rs6706648 | F | acgtcgtgaaagtctgacaaCTTGCCTCCCCCTGAC | 36 | 0.05 | G/A |
HBS1L-MYB | Â | Â | Â | Â | Â |
rs28384513 | F | aggtgccacgtcgtgaaagtctgacaaTACCTACGCCAGCGTTC | 44 | 0.15 | T/G |
rs7776054 | F | actaggtgccacgtcgtgaaagtctgacaaCAATATTTGTAATTTGTGTTCTGCTTCTAC | 60 | 0.1 | A/G |
rs9399137 | F | CAATAATGTAATTAACTGAACATATGGTTATT | 32 | 0.15 | T/C |
rs9389268 | F | GATTACAGGCGCATGCAACC | 20 | 0.3 | A/G |
rs4895441 | R | actgactaaactaggtgccacgtcgtgaaagtctgacaaCTTACTCAGTTCTCTGCTCATGTA | 63 | 0.1 | A/G |
rs6929404 | F | gtgaaagtctgacaaAAAAGTCTAGAGCACAAAAATTAAAATAA | 44 | 0.1 | C/A |
rs9402686 | F | GTTTAAAGTGTGTGACCTTGAGAC | 24 | 0.05 | G/A |
rs1320963 | R | agtctgacaaCTGAGAGTTCTTTTGGGATCTTTCAC | 36 | 0.05 | A/G |
rs6904897 | R | tcaggtgccacgtcgtgaaagtctgacaaGTGTATTTCTTTTGGTTGTGCATACA | 55 | 0.1 | T/G |
rs35959442 | R | gtctgacaaGCTCTTATAGCAGTCTACAGCAG | 32 | 0.05 | C/G |
rs9376090 | F | AGTCTAGCTGAGTGTTAGCC | 20 | 0.05 | T/C |
rs4895440 | F | GCGTGGCTGGGGAAAG | 16 | 0.1 | A/T |
rs9494142 | F | ccacgtcgtgaaagtctgacaaCAGTCAATTCGATTCTACTACTGACA | 48 | 0.05 | T/C |
rs9402685 | F | ccccccaactgactaaactaggtgccacgtcgtgaaagtctgacaaATGTTTCACCGTGTTGCTCAGG | 68 | 0.1 | T/C |
rs11759553 | F | aaagtctgacaaTGATTGGGGTAGGCCATAGG | 32 | 0.05 | A/T |
rs6934903 | F | tcaggtgccacgtcgtgaaagtctgacaaCCTGCATAAGTGTCGAATCTCTA | 52 | 0.05 | T/A |