Impact of CNV on CYP51 expression. (A) Bar graph presenting qRT-PCR data for the relative expression of EnCYP51 in sequenced isolates at 1 and 5 days post inoculation. Expression values are presented as fold-EnEF1 (number of EnCYP51 molecules/number of EnEF1 molecules), calculated using the ΔCt method as described in . Data were analyzed using a two-way ANOVA. Values are the mean ± standard error of three biological replicates. Different letters represent significant results (Tukey’s HSD test: P < 0.01). (B) Scatterplot showing the correlation between relative expression of EnCYP51 by qRT-PCR and copy number predicted by sequencing coverage. A linear trend is shown.