Syntenic and protein functional domain analysis of YTHDC2L1 and YTHDC2L5. (A) Schematic representation of conserved chromosomal loci in avian and non-avian vertebrate species showing the relative position of YTHDC2L1 (in red), a novel expansion of YTHDC2 that is only present in songbirds. Adjacent genes are indicated in black. The chromosome or scaffold number is indicated beneath each species common name. (B) An alignment of the protein family domains predicted for amino acid sequences derived from Chicken YTHDC2, the orthologous YTHDC2 “parent” gene in Zebra finch, as well as the copies of YTHDC2 that are only present in songbirds (YTHDC2L1), and Zebra finch (YTHDC2L5). Specific protein family domains predicted by InterProScan5 are aligned relative to Chicken YTHDC2, and are indicated by the various coloured symbols. YTHDC2 and YTHDC2L1 lack nearly all of the major protein family domains that are characteristic of YTHDC2. In contrast, YTHDC2L5 appears to be a nearly complete copy of YTHDC2.