Differential expression of YTHDC2L1 in song nucleus LMAN of the adult male Zebra finch brain. (A) A schematic depicting a sagittal brain section ~2 mm from the midline shows the approximate location of the in situ photomicrographs presented in panel B. (B) Photomicrograph of in situ hybridization of songbird novel gene YTHDC2L1 shows discrete expression in song nucleus LMAN. (C) Detailed view of this section reveals that expression of YTHDC2L1 is restricted to large cells of LMAN, with labelled foci evident in some cells pairs within cellular nuclei (inset). (D) A comparable view of paralogous gene YTHDC2L5 shows low levels of expression, non-differential in LMAN. Anatomical abbreviations: A, arcopallium; H, hyperpallium; HVC, proper name; LMAN, lateral magnocellular nucleus of the nidopallium; M, mesopallium; MD, dorsal mesopallium; MV, ventral mesopallium; N, nidopallium; RA, robust nucleus of the arcopallium; St, striatum. Scale bars: 100 μm in B–D; 20 μm in C inset.