Expression of miR168 and AGO1 in Arabidopsis plants treated with elicitors from F. oxysporum . (A) Stem-loop RT-qPCR analysis of miR168. RNAs were prepared from Arabidopsis plants treated with fungal elicitors for the indicated periods of time, and from control mock-inoculated plants (RNAs from samples harvested at 5, 30, 60 and 120 min were also used for microarray analysis). (B) qRT-PCR analysis of AGO1 using Ubiquitin10 (At5g65080) as the internal control. RNAs samples were the same as in (A). Results shown are from one of three independent experiments that gave similar results. Erro bars show the standard error. Asterisks indicate a significant difference between conditions (*, P ≤ 0.05 ; **; ≤0.01). c, control plants. e, elicitor-treated plants.