Figure 2

MiR168 activity in Arabidopsis plants revealed by GFP fluorescence patterns tissues of GFP -miR168* sensor plants. (A) Schematic representation of the miR168 sensor construct containing the GFP mRNA with a site complementary to miR168 (GFP-miR168*). (B) Plants constitutively expressing the GFP gene. Ten day-old plants were treated for 30 min with elicitors obtained from the fungus F. oxysporum. Water was used as mock control. Results obtained in elicitor-treated GFP-Arabidopsis plants are presented (similar patterns were observed in non treated GFP-Arabidopsis plants). GFP fluorescence images are shown. (C) Analysis of miR168 activity in control, non treated GFP-miR168* plants (sde1 background). Bright-field (left) and GFP fluorescence (right) images are shown. (D) miR168 activity in elicitor-treated GFP-miR168* Arabidopsis plants. Bright-field (left) and GFP fluorescence (right) images are shown. No GFP fluorescence was evident in roots of non treated plants due to miR168 guided silencing.