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Table 5 Primer set r 2 values and efficiencies, along with the length of each amplicon and primer

From: RNA-seq analysis of broiler liver transcriptome reveals novel responses to high ambient temperature

Primer Length Product size r2 Efficiency
SPSB4 For 20 bp 120 bp 1 100%
SPSB4 Rev 21 bp
TRPC5 For 20 bp 112 bp 1 104%
TRPC5 Rev 20 bp
BNC1 For 20 bp 133 bp 1 109%
BNC1 Rev 20 bp
ANGPTL4 For 20 bp 126 bp 1 91%
ANGPTL4 Rev 20 bp
LIMS2 For 20 bp 143 bp 1 100%
LIMS2 Rev 20 bp
ERC2 For 20 bp 149 bp 1 104%
ERC2 Rev 20 bp
S100A1 For 20 bp 120 bp 1 95%
S100A1 Rev 20 bp
FGF14 For 20 bp 129 bp 1 109%
FGF14 Rev 20 bp
DIO3 For 20 bp 143 bp 1 104%
DIO3 Rev 20 bp