Table 2 Primers used
Experiment | Aim | Name | sens | 5′-3′ sequence |
|---|---|---|---|---|
THI3 allelic switch | kanMX insert | THI3_i_f | Forward | GAAAGAACATAACTACTAAAACGCACCGTCGTCATTCTGAAGATGTTTCGTACGCTGCAGGTCGAC |
THI3_i_r | Reverse | GGTAATCATGAGGGTCCCTGGTAGTAGGGCGGAGAGATCAGATCAGCATAGGCCACTAGTGGATCTG | ||
THI3-S288c insert | THI3_A | Forward | TGCATTTGTCTTCTGCACACA | |
THI3_D | Reverse | TACAAAGTTGCGTGGTATCATCTTA | ||
qPCR | SCR1 | sCR1_Cf | Forward | TCTGGTGCGGCAAGGTAGTT |
sCR1_Cr | Reverse | CACCTTTGCTGACGCTGGAT | ||
UBC6 | UBC6_q_F | Forward | GGCTACAAAGCAGGCTCACAA | |
UBC6_q_R | Reverse | TGGAGGGTTTTCCACCATCA | ||
THI11 | THI11-qF | Forward | GGTGAATGGTTCGTCCAACAA | |
THI11-qR | Reverse | CAAGGCCCGTGGTTTCC | ||
PDC1 | PDC1-qF | Forward | CAGCACCCAAGGTAGCACCA | |
PDC1-qR | Reverse | AAACCACTTTCCCAAACAACACC | ||
PDC5 | PDC5-qF | Forward | AAGACTTGGACGATAGCGTATACATC | |
PDC5-qR | Reverse | CCAGCTAGAGTTCCAATTACCAAGT |