Correlation of fold changes using FFPE vs FF tumors. The tumors from our institutional biobank were high risk tumors. To calculate expression fold changes between high risk and low risk tumors, low risk tumors (all FF tumors) were identified from The Cancer Genome Atlas (TCGA). Genes highly expressed by RNA-seq in both our institutional tumors and TCGA tumors were used to calculate fold change. The correlation between fold changes determined from FFPE and FF tumors is shown. The fold change was calculated using log2 FPKM. HR, high risk tumors from our institution; LR, low risk tumors from TCGA; FPKM, fragments per kilobase of exon per million fragments mapped.