Table 2 Primers used on RNA-Seq validation
From: Flower development and sex specification in wild grapevine
Gene/annotationa | Chromosome | Primers (Forward/Reverse) | Product size |
|---|---|---|---|
VvAP1 (Vv01s0011g00100) | 1 | 5’ – GAACAAGATCAATCGCCAAG – 3’ | 128 bp |
5’ – ACAGCTTTCCTTTAGTGGAG – 3’ | |||
VvAP3 (Vv18s0001g13460) | 18 | 5’ – GAATTTGATGCAAGGGACAG – 3’ | 169 bp |
5’ – TAAAGGTCAAATCAGAGCCC – 3’ | |||
VvLfy (Vv17s0000g00150) | 17 | 5’ – GAGAGGCAGAGGGAGCATCC – 3’ | 210 bp |
5’ – GCTTGCTCCCGCCTTCTTCGC – 3’ | |||
VvTFL (Vv06s0080g00290) | 6 | 5’ – GTTGGTAGAGTGATTGG – 3’ | 317 bp |
5’ – GAAATGCCAAGGCCAAATAT – 3’ | |||
VvPi (Vv18s0001g01760) | 18 | 5’ – GGAGAATGATAGCATGCAGA – 3’ | 254 bp |
5’ – TTTCCACCTCTCTCACATTG – 3’ |