Figure 2

Percentage of SASI fragments detected after different Covaris shearing regimes. With all shearing conditions, some intact A, B and C fragments with barcode number 1 (here referred to as A1-R1, B1-R1 or C1-R1) are detected. In general the more intense the shearing regime, the greater number of partial, or broken amplicons (fragments with terminal sequences A1, B1, C1 or R1 at one end and an internal sequence at the other end, here referred to as A1-broken, B1-broken, C1-broken or R1-broken), are detected. The longer amplicons are more susceptible to breakage during shearing than the smallest A1-R1 amplicon (214 bp).