Figure 1

Comparison of unigene expression as Reads Per kb per Million (RPKM). Expression level was determined using the RPKM method, thereby eliminating the influences of gene length and sequencing discrepancies on the calculation of gene expression. A, RPKM with 102,857 unigenes, B, RPKM after selection based on FDR ≤ 0.001 AND |log₂Ratio| ≥ 1with 3,977 unigenes. Va-24 hr: RPKM at 24 hours after infection with V. alginolyticus, TSB (tryptic soy broth)-24 hr: RPKM at 24 hours after injection with TSB.