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Figure 1 | BMC Genomics

Figure 1

From: Unbiased chromatin accessibility profiling by RED-seq uncovers unique features of nucleosome variants in vivo

Figure 1

The RED-seq method for genome-wide measurement of RE accessibility. (A) RED-seq workflow. RSs are shown in red, yellow boxes (Step 3) represent RS-proximal adaptors, dark blue boxes (Step 5) represent RS-distal adaptors, orange circles represent biotin, light blue boxes represent paired-end PCR primers, large blue circles (Step 1) represent nucleosomes, and DNA is shown in black. (B) Ethidium bromide stained agarose gel indicating bulk digestion levels of chromatin and naked DNA. (C) An example FASTQ file is shown to illustrate the near-uniform sequencing of the RS-containing end of each fragment in the library, signified by the large enrichment of G at position 5, and a CC dinucleotide at positions 7 and 8, derived from the cleaved and blunt-ended Sau96I site (GNCC).

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