Figure 1From: Genes associated with thermosensitive genic male sterility in rice identified by comparative expression profilingPhenotypes and OsUgp1 expression of H1493 and TGMS-Co27 plants grown at high and low temperature. A. Phenotypes of TGMS-Co27 plants grown at high temperature (left) and low temperature (right). B. Phenotypes of H1493 plants grown at high temperature (left) and low temperature (right). C. Results of Western blotting analysis of the abundance of UGPase proteins in florets of H1493 and TGMS-Co27 plants grown at high temperature and low temperature. D. Results of qPCR analysis of OsUgp1 gene expression in the florets. Total Ugp1 mRNAs include endogenous and transgenic Ugp1 mRNAs. E. Semi-quantitative RT-PCR analysis of the expression of the correctly spliced transgenic Ugp1 mRNAs. The presence of equal quantities of template in each reaction was verified by amplifying a constitutively expressed actin. H1493HT and H1493LT refer to H1493 plants grown at high temperature and low temperature, respectively. Co27HT and Co27LT refer to TGMS-Co27 plants grown at high temperature and low temperature, respectively.Back to article page