Figure 7

Quantitative expression analysis of alternative splicing variants by real-time RT-PCR. The investigated sample genes with alternative splicing were clpr2 (A), cyn23 (B), hyd2 (C), oee1 (D), lsg2 (E) and ppi1 (F). Primers were designed to amplify a characteristic fragment of each alternative splicing variant specifically (Figure 4 and Additional file 1: Table S3). On the left side of each panel, the expression levels of both alternative splicing variants are shown in relation to the actin gene (the expression level of actin was set as 100%). On the right side of each panel, the expression levels of both alternative splicing variants are compared against each other (the expression level of V1 was set as 100%).