Validation of part of differentially expressed unigenes by qRT-PCR. (A) down-regulated unigenes in treated fruit. C7592: aldehyde dehydrogenase; C16397: aldehyde dehydrogenase family 2 member B7; C1509: NADH dehydrogenase; C82323: Transcription factor bHLH49; C493: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1; C70483: 3-dehydroquinate dehydratase/shikimate 5-dehydrogenase; C3862: Diospyros kaki anthocyanidin synthase; C40762: D. kaki flavonoid 3'5' hydroxylase; C6120: VTC2-like protein; C1104: D. kaki DkMyb2 mRNA for putative MYB transcription factor; C76085: D. kaki DKSCPL1 mRNA for serine carboxypeptitase-like protein 1; C586: DKSCPL1 mRNA for serine carboxypeptitase-like protein 1. (B) up-regulated unigenes in treated fruit. C2282: D. kaki MADS-box protein (MADS1) mRNA; C16427: dehydroascorbate reductase 1; C13761: aldehyde oxidase; C14205: D. kaki DkSK mRNA for putative shikimate kinase; C772: WD-repeat protein; C67321: 4-hydroxyphenylpyruvate dioxygenase; C15698: glutathione peroxidase; C18687: alcohol dehydrogenase (Adh3) mRNA; C21926: D. kaki beta-carotene hydroxylase mRNA; C66509: tetrahydroxychalcone 2'-glucosyltransferase; C11332: tonoplast intrinsic protein; C19374: ferulate 5-hydroxylase (F5H) mRNA.