Figure 4

Binding of MrpC2 to candidate genes from ChIP-seq. For the indicated genes, approximately 200 bp of DNA surrounding a peak from the ChIP-seq analysis was amplified by PCR with ³²P-labeled primers. These probes (2 nM) alone (-) or after addition of His₁₀-MrpC2 (+) (1 μM, except lane 4 in which 0.5 μM was added) were subjected to EMSAs. MXAN_5802 and 0524 were analyzed on the same gel, with intervening lanes removed, as indicated by narrower separation between panels. Filled and open arrowheads indicate complexes inferred to have one or two His₁₀-MrpC2 bound, respectively.