Figure 6

Patterns of MrpC2 and FruA binding to candidate genes from ChIP-seq. For the indicated genes, approximately 200 bp of DNA surrounding a peak from the ChIP-seq analysis was amplified by PCR with ³²P-labeled primers. The MXAN_0524 probe contained only the upstream predicted MrpC2 binding site. The DNA probes (2 nM) were incubated with His₁₀-MrpC2 (1 μM) and/or FruA-His₆ (3 μM) as indicated (unless noted below), and subjected to EMSAs. A lower concentration of His₁₀-MrpC2 (0.03 μM in lanes 5 and 6; 0.1 μM in lanes 12, 14, 19, and 20) and FruA-His₆ (1.5 μM in lanes 13 and 14) was used in some experiments. Filled arrowheads pointing leftward or rightward are inferred to indicate complexes with one or more FruA-His₆ bound, respectively. Brackets and open arrowheads indicate novel or more abundant complexes produced by the combination of proteins than by either protein alone. Panels with a narrower separation were analyzed on the same gel, with intervening lanes removed.