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Table 2 The information of primers used in qPCR

From: Copy number variations of the extensively amplified Y-linked genes, HSFY and ZNF280BY, in cattle and their association with male reproductive traits in Holstein bulls

Primer name

Sequence

Annealing Tm

Primer efficiency

HSFYF

5′-CTCCTAAGGATGAATCAACTG-3′

59°C

1.88

HSFYR

5′-CACAAGATCCTCAGACAAAGC-3′

ZNF280BYF

5′-GAAATACCACACCACCTGCC-3′

64°C

1.88

ZNF280BYR

5′-GATCTGTAACTGCAAACCTGG-3′

DDX3YF

5′ -ATCGTGGGCGGAATGAGTGT-3′

59°C or 64°C

1.95 at 59°C or 1.90 at 64°C

DDX3YR

5′ -CTTGGTGGAAGCGGTTTTGA-3′