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Table 2 The information of primers used in qPCR

From: Copy number variations of the extensively amplified Y-linked genes, HSFY and ZNF280BY, in cattle and their association with male reproductive traits in Holstein bulls

Primer name Sequence Annealing Tm Primer efficiency
HSFYF 5′-CTCCTAAGGATGAATCAACTG-3′ 59°C 1.88
HSFYR 5′-CACAAGATCCTCAGACAAAGC-3′
ZNF280BYF 5′-GAAATACCACACCACCTGCC-3′ 64°C 1.88
ZNF280BYR 5′-GATCTGTAACTGCAAACCTGG-3′
DDX3YF 5′ -ATCGTGGGCGGAATGAGTGT-3′ 59°C or 64°C 1.95 at 59°C or 1.90 at 64°C
DDX3YR 5′ -CTTGGTGGAAGCGGTTTTGA-3′