qRT-PCR validation of chilling-responsive conserved and novel miRNAs in ‘LA1777’ (a) and ‘Hezuo908’ (b) tomato. The reference gene was 5S rRNA. All reactions were repeated three times. Normalized miRNA expression amount at 1 h without chilling treatment was arbitrarily set to 1. Differences between the NT and CT libraries were tested with a T test. *indicates a significant difference between the two libraries (<0.05).