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Table 1 Statistics of sRNA sequences from the CT and NT libraries

From: Identification of chilling stress-responsive tomato microRNAs and their target genes by high-throughput sequencing and degradome analysis

Type

NT

CT

Number of total sRNA

Percentage of total (%)

Number of unique sRNA

Percentage of unique (%)

Number of total sRNA

Percentage of total (%)

Number of unique sRNA

Percentage of unique (%)

Raw reads

9,919,778

100.00

2,280,543

100.00

6,199,678

100.00

1,521,423

100.00

3ADT&length filtera

1,432,000

14.44

333,989

14.65

1,009,234

16.28

196,123

12.89

Junk reads

27,989

0.28

14,986

0.66

14,948

0.24

8,225

0.54

Rfamb

1,181,057

11.91

118,282

5.19

795,804

12.84

95,041

6.25

mRNA

4,132

0.04

2,480

0.11

3,320

0.05

2,007

0.13

Repeatsc

63,383

0.64

20,334

0.89

50,626

0.82

16,844

1.11

rRNA

860,830

8.68

72,264

0.73

524,172

8.45

53,070

0.86

tRNA

197,083

1.99

19,182

0.19

184,888

2.98

19,919

0.32

snoRNA

17,886

0.18

5,419

0.05

17,466

0.28

4,893

0.08

snRNA

15,389

0.16

5,618

0.06

12,882

0.21

4,598

0.07

other Rfam RNA

89,869

0.91

15,799

0.16

56,396

0.91

12,561

0.20

Clean reads

7,231,609

72.90

1,795,513

78.73

4,342,604

70.05

1,208,259

79.42

  1. areads lacking three ADTs or with lengths <17 nt or >25 nt were removed.
  2. bcollection of many common noncoding RNA families other than micro RNAs; http://rfam.janelia.org.
  3. cdownloaded from http://www.girinst.org/repbase.