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Table 2 Effects of sequence improvement on the annotation of R-genes

From: Interspecific and intraspecific gene variability in a 1-Mb region containing the highest density of NBS-LRR genes found in the melon genome

Gene Id1

Class2

Sequence changes3

Notes

MELO3C004258

TN

No

3′-partial

MELO3C004259

TNL

(T)14 -- > (T)11 (I)

 

(T)10 -- > (T)9 (I)

 

MELO3C004260

TN

No

3′-partial

MELO3C004262

TNL

(T)7 -- > (T)8 (I)

Additional a.a.s (gap closed)

(T)4 -- > (T)3 (I)

1 T deleted (I)

20 N - > [559 bp (I + E) + 30 N (in poly(AT), I)]

MELO3C004266

TNL

(N)5054 gap closed (E)

Additional a.a.s (gap closed)

(CTATAATTG) -- > (CATTG) (E)

(TGTCGTTTA) -- > (T9GCGTTA) (I)

(TTATA9T) -- > (TA9T) (I)

(A6CA5G) -- > (A7CA6G) (I)

MELO3C004288

N

No

5′- and 3′-partial

MELO3C004289

TNL

(N)767 gap closed (E)

Additional a.a.s (gap closed)

(A)20 -- > (A)26 (I)

(A)16 -- > (A)24 (I)

(T)7(A)11 -- > (T)8(A)1o (I)

MELO3C004290

TN

(N)20 - > (N)1 (E)

3′-partial

MELO3C004291

TN

(N)2153 gap closed (E)

 

(CA5CCT12) -- > (CAAT10) (I)

3′-partial

(CTTTTA13) -- > (CTTTA11) (I)

Additional a.a.s (gap closed)

(GAAAG) -- > (GAG) (E)

 

MELO3C004292

TNL

(A)14 -- > (A)7 (I)

 

(AA) deleted (I)

 

(A)21 -- > (A)15 (I)

 

MELO3C004294

TNL

(AA) deleted (I)

 

MELO3C004301

TNL

(N)73 - > (N)28 (I)

(N)28 in poly(TA) (intron)

(TACCA28GAAA) -- > (TCCA28GA) (I)

(A10CCAAAG) -- > (A13CCAAAAG) (I)

(AAAAGTTTA6) -- > (AAAAGTTTCA6) (I)

(GAAAGTATA5TCA) -- > (GAAAGTATAAATCA) (I)

(TTA10TTTGAAAA) -- > (A11TGAAAA) (I)

MELO3C004302-4303 4

NL

(CTTGAATGAAACTTA) -- > (CTTGAAC) (E)

5′-partial5

(GTGAAACTTA11CATGG) -- > (GTA8CATGG) (I)

(TACAATT) -- > (TACTT) (E)

(CTA11CAT) -- > (CTA8CAT) (E)

MELO3C004309-4310 6

TNL

(N)78 gap closed (I)

 

(GTTCCGTATTCTAATTAT4ACT3) -- > (GTCCGATCAATAT3ACT) (I)

 

(TAT6C) -- > (TATTC) (E)

 

MELO3C004311

TN

(N)30 gap closed (E)

3′-partial

(GA4TTTACT) -- > (GA5TACT) (I)

MELO3C004312

TN

(N)468 gap closed (introns + exons)

3′-partial

Additional a.a.s (gap closed)

MELO3C004313

TN

(N)20 gap closed (E)

3′-partial

Additional a.a.s (gap closed)

MELO3C004317

CNL

(N)1414 gap closed (E + I)

Additional a.a.s (gap closed)

(N)4442 gap closed (exons + introns)

MELO3C004318

CN

(N)4962 gap closed (exons + introns)

3′-partial

Additional a.a.s (gap closed)

MELO3C004320

NL

(N)4489 -- > (N)2460 (introns + exon)

Add. a.a.s (gap partially solved)

MELO3C004321

CNL

(N)3747 gap closed (E)

Additional a.a.s (gap closed)

MELO3C004323

CN

No

3′-partial

MELO3C004324

NL

No

 
  1. 1MELO3C004261, MELO3C004265, MELO3C004295, MELO3C004304, MELO3C004319 are ORFs that code for, respectively, 141-, 220-, 98-, 109-, and 233-aa peptides with homology to TNL proteins. Due to their short length, they are most probably not R-genes and, therefore, were not considered for further analysis.
  2. 2TN: TIR-NBS; TNL: TIR-NBS-LRR; N: NBS; NL: NBS-LRR; CNL: CC-NBS-LRR; CN: CC-NBS.
  3. 3Sequence changes between assemblies CM3.5 and CM3.6.1 in each R-gene region (start to stop codon, including introns). I, intron; E, exon.
  4. 4Previous MELO3C004302 gene does belong to the MELO3C004303 ORF, as deduced by sequence comparison with other TNL R-proteins.
  5. 5Gene MELO3C004304 codes for a 109-aa peptide with a TIR domain, and may represent a 5′ fragment of gene MELO3C004302-4303.
  6. 6Previous MELO3C004310 gene does belong to the MELO3C004309 ORF, as deduced by sequence comparison with other TNL R-proteins.