Figure 2
From: Investigation of protein secretion and secretion stress in Ashbya gossypii
Glucose consumption ( solid lines ) and growth ( dashed lines ) of recombinant A. gossypii in batch cultures in AFM with G418 at pH 6.0, 30°C and 500 rpm, with 1.0 vvm aeration. (○) EGI producing strain, (▲) empty vector strain and (●) DTT-stressed EGI producing cells. Values represent the average ± standard deviation of two independent bioreactor cultures. Grey arrows indicate the sampling times for non-treated cultures and black arrows the sampling points for DTT-treated cultures. *Indicates the time at which DTT was added to EGI producing cultures.