Figure 4

Acetate formation from H ₂  + CO ₂ by resting cells of T. kivui is inhibited by TCS. Whole cells of T. kivui were incubated with H₂ + CO₂ in buffer containing 50 mM imidazole, 20 mM MgSO₄, 20 mM KCl, 50 mM KHCO₃ and 4 mM DTE (pH 7.0). A: 350 ± 20 μM (■) or 20 mM (▲) NaCl; B: 20 mM NaCl with (▼) or without (▲) 30 μM ETH2120; C: 350 ± 20 μM NaCl with (♦) or without (■) 30 μM TCS. The final protein concentration of the resting cells in the assay was 1 mg/ml. All values are mean from three replicates.