Figure 5

EMSA for Bxe_B3018 and possible effectors. EMSA examination of aminoacetone (Aa), methylglyoxal (Mgx) and aminopropan-2-ol (Ap) as potential disruptors of DOS/GST-Bxe_B3018 complex formation. Lane 1 contained unbound DOS (1 μM) as a control and lane 2 contained the DOS/TR complex control (10 μM TR and 1 μM DOS, the standard concentrations for each lane in this experiment). 10, 15 and 20 mM were the concentrations used for effectors. Lanes 3–5 show the simultaneous incubation of TR and its DOS in the presence of increasing concentrations of aminoacetone, lanes 6–8 with methylglyoxal and lanes 9–11 with aminopropan-2-ol. The image show here is representative of a single 20-lane gel, with empty lanes omitted at the dotted lines.