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Figure 1 | BMC Genomics

Figure 1

From: Complex coding of endogenous siRNA, transcriptional silencing and H3K9 methylation on native targets of germline nuclear RNAi in C. elegans

Figure 1

Loss of HRDE-1 increases RNA polymerase II (Pol II) occupancy at specific genomic loci. (A) Comparison of averaged Pol II occupancy levels in the wild type and hrde-1(tm1200) mutant animals around the 5′ and 3′ ends of 3903 “H3K4me2/3-anchored” genes [44]. Left panels: Pol II ChIP or ChIP input signal was plotted as a function of distance to the dyad of the “plus-one” nucleosome, defined by peak H3K4me2/3 nucleosomes near transcription start sites for the 3903 ‘H3K4me2/3-anchored’ genes. Right panels: Pol II ChIP or ChIP input signal was plotted as a function of distance to the annotated ends of 3′ UTR. (B) Pol II ChIP or ChIP input signals for the wild type and hrde-1(tm1200) mutant animals on 100,257 1-kb regions in the C. elegans genome. Each dot corresponds to a 1-kb region. Dotted lines indicate no-changes (the middle line) and three-fold differences (the flanking lines).

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