Loss of HRDE-1 increases the levels of pre-mRNA and mRNA transcribed from the GRTS regions. (A) Percentages of sequenced tags (pre-mRNA-seq or mRNA-seq) that match to sequences of exons or introns. Scatter plots of the pre-mRNA-seq (B) or mRNA-seq signals (C) for the wild type and hrde-1(tm1200) mutant animals. (D-F) Scatter plots of the mRNA-seq signals for the wild type, nrde-2(gg91), nrde-3(gg66) and nrde-4(gg129) mutant animals. The percentage of the exemplary HRDE-1 targets showed at least 3-fold increases in mRNA-seq signals in each mutant was indicated in each panel.