HRDE-1 is not required for the biogenesis of germline nuclear RNAi-associated endo-siRNA. (A) Scatter plots of the small RNA-seq signals for the wild type and hrde-1(tm1200) mutant animals. Each dot represents each of the 100,257 1-kb regions, with GRTS, GRH, and overlapped regions between GRTS and GRH highlighted. Dotted lines indicate no-changes (the middle line) and three-fold differences (the flanking lines). (B) The size distribution and nucleotide distribution for the first position at the 5′ end of small RNAs in the GRTS and GRH regions.