Figure 2

Using more siRNAs adds power and yields reproducible results. (A) The three boxplots show Pearson correlation coefficients R between screens performed using the same siRNA set. The numbers 1 to 3 correspond to the total number of replicate screens that we averaged and compared to another distinct set of replicate screens, averaged over the same number. We resampled the replicate screens up to 500 times. The scatter plot shows an example for the correlation of infection indices from the duplicate of Adenovirus Dharmacon pooled screen. (B) The set of six boxplots shows the Pearson correlation coefficients of the averaged readouts from 1 to 6 siRNA sets. The scatter plots depict the correlation of infection indices for Adenovirus, the first between two different single siRNAs and the second between each an average over six siRNAs.