Statistics on used siRNA libraries and hits. (A) We weighted siRNAs based on their library quality. Each vertical compartment in the plot corresponds to a training set of siRNAs. We averaged data in the training set from the siRNAs of the specific manufacturers. Each boxplot corresponds to a test set of single siRNAs from different manufacturers (except “Dharm. siRNA mean” which is the average of 4 Dharmacon unpooled siRNAs). Y-axis refers to Pearson correlation coefficients R between the training and test sets. A star corresponds to significant differences in the correlation coefficients (Mann–Whitney-U-test p < 0.05) between pairs of manufacturers. We used all screens, infection index, and cell number well readouts in the analysis. We used the results of this analysis to assign siRNA weights to siRNAs from different library manufacturers as shown below the plot. (B) The histogram shows obtained FDR q-values from all screens using the infection index readouts. The red line shows the FDR-threshold of 0.4. (C) The bar shows number of up and down hits for different pathogens. (D) The bar plot shows the number of hit genes that were shared between pathogens.