Skip to main content

Table 1 Recombination frequency in the genetic cross between VAND and ME49

From: NextGen sequencing reveals short double crossovers contribute disproportionately to genetic diversity in Toxoplasma gondii

Number of rarasitesa No drugsb FUDRc SNFd FUDR and SNFe Calculations
10,000 parasites 96 70 80 10  
10,000 parasites 104 70 66 18  
10,000 parasites 120 72 90 13  
  106.67 70.67 78.67 13.67 Average
  12.22 1.15 12.05 4.04 S.D.
   12.81% Recombination Frequencyd   
  1. atotal number of parasites added to 6 well plate. Although the overall viability in this assay was low, the consistency across three replicates suggests that this did not affect the outcome.
  2. bnumber of plaques formed in the absence of drugs at 7 days post-infection.
  3. cnumber of plaques formed in the presence of fluorodeoxyuridine (FUDR, 3 × 10-5 M) at 7 days post-infection.
  4. dnumber of plaques formed in the presence of synefungin (SNF, 3 × 10-7 M) at 7 days post-infection.
  5. enumber of plaques formed in the presence of both SNF and FUDR at 7 days post-infection.
  6. dCalculation of recombination frequency = number of plaques formed in the presence of both drugs / the number of plaques formed in the absence of drugs × 100.