NextGen sequencing reveals short double crossovers contribute disproportionately to genetic diversity in Toxoplasma gondii

Table 1 Recombination frequency in the genetic cross between VAND and ME49

Number of rarasites^a	No drugs^b	FUDR^c	SNF^d	FUDR and SNF^e	Calculations
10,000 parasites	96	70	80	10
10,000 parasites	104	70	66	18
10,000 parasites	120	72	90	13
	106.67	70.67	78.67	13.67	Average
	12.22	1.15	12.05	4.04	S.D.
		12.81%	Recombination Frequency^d

^atotal number of parasites added to 6 well plate. Although the overall viability in this assay was low, the consistency across three replicates suggests that this did not affect the outcome.
^bnumber of plaques formed in the absence of drugs at 7 days post-infection.
^cnumber of plaques formed in the presence of fluorodeoxyuridine (FUDR, 3 × 10^-5 M) at 7 days post-infection.
^dnumber of plaques formed in the presence of synefungin (SNF, 3 × 10^-7 M) at 7 days post-infection.
^enumber of plaques formed in the presence of both SNF and FUDR at 7 days post-infection.
^dCalculation of recombination frequency = number of plaques formed in the presence of both drugs / the number of plaques formed in the absence of drugs × 100.

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