From: Changes in activity of metabolic and regulatory pathways during germination of S. coelicolor
Functional group | Deactivation in t1-t2 spore rehydration | Activation in t2-t3 | Activation in t7-t8 first DNA replication | Other periods | Note |
---|---|---|---|---|---|
Amino acid metabolism pathways | 250 Alanine, aspartate and glutamate | 250 Alanine, aspartate and glutamate | Â | Â | |
260 Glycine, serine and threonine | 260 Glycine, serine and threonine | ||||
270 Cysteine and methionine | 270 Cysteine and methionine | ||||
280 Valine, leucine and isoleucine degradation | 280 Valine, leucine and isoleucine degradation | ||||
290 Valine, leucine and isoleucine biosynthesis | 290 Valine, leucine and isoleucine biosynthesis | ||||
300 Lysine biosynthesis | 300 Lysine biosynthesis | ||||
310 Lysine degradation | 330 Arginine and proline | ||||
330 Arginine and proline | 340 Histidine400 Phenylalanine, tyrosine and tryptophan biosynthesis | ||||
340 Histidine | |||||
350 Tyrosine 360 Phenylalanine | |||||
380 Tryptophan | |||||
400 Phenylalanine, tyrosine and tryptophan biosynthesis | Â | ||||
Carbohydrate metabolism | TCA cycle/pentose phosphate/glycolysis/gluconeogenesis | TCA cycle/pentose phosphate/glycolysis pathways | TCA cycle-associated pathways and genes. | Deactivated in t7-t8 | the majority - 23 of 57 were associated with the TCA cycle/pentose phosphate/glycolysis pathways genes |
40 Pentose and glucuronate interconversions | 630 Glyoxylate and dicarboxylate metabolism | 40 Pentose and glucuronate interconversions (2) | 10 of 30 of the TCA cycle-associated pathways | ||
51 Fructose and mannose metabolism | 640 Propanoate metabolism | 51 Fructose and mannose metabolism | 52 Galactose metabolism | ||
500 Starch and sucrose metabolism | 650 Butanoate metabolism | 500 Starch and sucrose metabolism | 500 Starch and sucrose metabolism | ||
520 Amino sugar and nucleotide sugar metabolism | 660 C5-Branched dibasic acid metabolism. | 52 Galactose metabolism | 520 Amino sugar and nucleotide sugar metabolism | ||
562 Inositol phosphate metabolism | 53 Ascorbate and aldarate metabolism | 562 Inositol phosphate metabolism | |||
630 Glyoxylate and dicarboxylate metabolism | 520 Amino sugar and nucleotide sugar metabolism | 650 Butanoate metabolism | |||
650 Butanoate metabolism. | 562 Inositol phosphate metabolism | 660 C5-Branched dibasic acid metabolism | |||
630 Glyoxylate and dicarboxylate metabolism | |||||
640 Propanoate metabolism | |||||
650 Butanoate metabolism | |||||
Energy metabolism | 190 Oxidative phosphorylation, | 190 Oxidative phosphorylation | 190 Oxidative phosphorylatio | genes associated with cytochrome C activated in t11-t12 | A minor portion of the genes with diminished expression at t7-t8 only, represented catalase (catA and B; SCO379 and 0666) and nitrate and nitrite reductases (SCO4947, 6102 and 6532) |
680 Methane | 680 Methane metabolism, | 680 methane metabolism | |||
910 Nitrogen metabolisms | 910 Nitrogen metabolism, | 910 nitrogen metabolism | |||
Folding, sorting and degradation | 20S Proteasome subunits (SCO1643 and 1644) and a Signal peptidase I (SCO5597 and 5598). | 3018 RNA degradation | modest activation of chaperone GroEL (SCO4296 and 4762) | DnaK and GroEL was for both diminished at t6-t7. | Â |
3060 Protein export | |||||
Lipid metabolism and membrane transport | Acetyl CoA carboxylase and acetyltransferase (SCO2445, 5399) |  |  | activation at (t1-t2) – |  |
561 Glycerolipid metabolism | 61 and 71 Fatty acid biosynthesis pathways | ||||
564 Glycerophospholipid metabolism | Deactivation at t7-t8 - ABC transporters,membrane transport system. | ||||
ABC transporters | |||||
genes of the secretion system | |||||
Nucleotide metabolism | Â | 240 Pyrimidine metabolism | Â | Â | Â |
230 Purine metabolism | |||||
Replication and repair | 3030 DNA replication | 3030 replication | |||
3410 Base excision repair | 3430 mismatch repair | ||||
3420 Nucleotide excision repair | 3440 homologous recombination | ||||
3430 Mismatch repair | |||||
3440 Homologous recombination | |||||
Transcription/translation | 970 Aminoacyl-tRNA biosynthesis | Â | Ribosomal proteins | Induced in the t2-t4 time interval. RNA polymerase complex genes, ribosomal proteins (L1, L2, L3, L4, L5, L6, L9, L10, L11, L14, L15, L16, L17, L18, L21, L22, L23, L24, L25, L30, S3, S4, S5, S6, S7, S8, S10, S12, S16, S18, S19) | Â |
ribosomal proteins | 970 Aminoacyl-tRNA biosynthesis |