Figure 4

Comparison of DNA copy numbers obtained using different techniques. DNA copy numbers of methylated/unmethylated DNA standards based on specifications of manufacturer (Expected), measured by flourometer and of p14 by Restriction Enzyme (RE) dPCR and p14 and COL2A1 with MethyLight dPCR in the 0% and 100% methylated samples. Copy numbers shown were obtained from 5 ng starting material (based on expected DNA quantity), pre-bisulfite conversion and RE digestion. RE dPCR data shows p14 copy number from the mock, MSRE and MDRE treatments. MethyLight dPCR data shows copy number obtained using the p14_M assay in singleplex and the methylation independent control COL2A1. Statistical comparisons are for Student’s t-test (* = p < 0.05; ns = not significant). Error bars show ± Standard Deviation of measurement.