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Table 3 MethyLight qPCR and dPCR

From: Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

Run no.

MID no.

% methylation

Expected (× replicate no.)

Observed

1

1

100

100

2

90

96.4

3

75

87.7

9

50 × 1

85.3

4

50 × 2

60.2

7

50 × 3

64.3

5

25 × 1

35.6

10

25 × 2

38.7

8

10

24.9

6

0

0.2

2

7

100

99.7

3

90

94.8

8

75

91.8

9

50 × 1

84.5

10

50 × 2

61.5

6

50 × 3

74.5

1

25 × 1

29.8

4

25 × 2

28.2

5

10

15.7

2

0

0.6

  1. The % methylation determined by bisulfite amplicon NGS using Multiplex Identifier (MID) fusion primers to amplify samples from a panel of methylated/unmethylated DNA standards mixed in a range of known ratios. The 25% and 50% methylated samples were replicated (× 1-3) within each of two runs.