Skip to main content

Table 3 MethyLight qPCR and dPCR

From: Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

Run no. MID no. % methylation
Expected (× replicate no.) Observed
1 1 100 100
2 90 96.4
3 75 87.7
9 50 × 1 85.3
4 50 × 2 60.2
7 50 × 3 64.3
5 25 × 1 35.6
10 25 × 2 38.7
8 10 24.9
6 0 0.2
2 7 100 99.7
3 90 94.8
8 75 91.8
9 50 × 1 84.5
10 50 × 2 61.5
6 50 × 3 74.5
1 25 × 1 29.8
4 25 × 2 28.2
5 10 15.7
2 0 0.6
  1. The % methylation determined by bisulfite amplicon NGS using Multiplex Identifier (MID) fusion primers to amplify samples from a panel of methylated/unmethylated DNA standards mixed in a range of known ratios. The 25% and 50% methylated samples were replicated (× 1-3) within each of two runs.