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Figure 7 | BMC Genomics

Figure 7

From: The translation initiation complex eIF3 in trypanosomatids and other pathogenic excavates – identification of conserved and divergent features based on orthologue analysis

Figure 7

Biochemical characterization of Leishmania EIF3. A Subcellular localization of Leishmania EIF3E. The experiment was carried out through indirect immunofluorescence using the affinity purified anti-EIF3E antibodies. Where indicated, the cells were counterstained with TOTO-3 to locate the nuclear and kinetoplast DNA. B Immunoprecipitation (IP) of native EIF3E. IP reactions were set up using total L. infantum cytoplasmic extract and the affinity purified antibodies directed against Leishmania EIF3E, as well as the respective pre-immune control serum (IP Control). Precipitated immunocomplexes were then used in Western blot assays with the same anti-EIF3E antibodies used for the IP. C Summary of the mass-spectrometry analysis of the precipitated samples. A total of 13 polypeptides are shown which were specifically co-precipitated with the anti-EIF3E antibodies. The parameters shown in the table used to determine specificity for the IP reactions using the anti-EIF3E antibodies, always compared with the control IPs, are described in the Methods section. EIF1: translation initiation factor sui1. G6PD: Glucose-6-Phosphate 1-Dehydrogenase.

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