Transcriptograms for technical and biological replicates with different windows sizes. Transcriptograms of technical replicates using a A) α = 1 ordered gene list and B) a randomly ordered gene list. Observe that both profiles are smoothened by window averages, indicating noise reduction. The transcriptogram obtained for the ordered list, however, conserves signal as compared to that a random ordered list is used. C) Transcriptograms for α = 1 and r = 80 for the six technical replicates of ρ0 cell line with modularity (r = 80) as the gray profile in the background. The horizontal red line is the expression global average. Observe that the trasncriptograms overlap almost perfectly. D) Average transcriptograms for α = 1 and r = 80 over sets of four technical replicates for each one of the five F cybrid biological replicates, relative to the average transcriptogram of all F cybrids (represented by the horizontal line at relative expression equal to 1). The colored shadows around each profile correspond to standard deviations. Observe the change in scale: the standard deviations are of the order of 1% of the average expression level. Since all colored regions overlap, the set of all 20 transcriptograms (biological and technical replicates) are not significantly different.