Table 5 Primer sequences for in situ hybridization probes
From: Analysis of the basal chordate Botryllus schlosseri reveals a set of genes associated with fertility
Gene symbol | Primers sequence used for each gene 5′-3′ |
|---|---|
hsd17β8 | Fwd-GCGGTCTGGTAAGCTTTGGT |
Rev-GTCGGACGCAAGGAACAAAC | |
selp | Fwd-CCTTCAGTTGCAACAAGGGC |
Rev-ACCGTTTCGGAGAGTTTCCC | |
tsk-2 | Fwd-AAAATGCGAGAACATCCTACTCA |
Rev-TTCTGTTAGGTCCCCTGATTGTA | |
otoa | Fwd-CGCAGCGCAGGATTAAACTC |
Rev-GCCGTATTTGTCGTGGATGC | |
vtg-1 | Fwd-CCGAACCGTACGGATACCTG |
Rev-TACGTTTTGGACGAAGGCGA | |
tspan-8 | Fwd-CAATTTGGCGCTGTTCCTCG |
Rev-CACGACTCTTACGCGTATCCA | |
zp-1 | Fwd-AGACGTGGTACCCATAGCCT |
Rev-ACGCTTATCGTGCAAGTGGA | |
ldlr | Fwd-GAATTTGCAGCTCGCTCTCG |
Rev-CATGACGAGTGCTCATCGGA | |
tsk-1 | Fwd-GGAAAATGCTCGGAACGGTG |
Rev-CCCGAAAAACGGATGTCCA | |
tll-1 | Fwd-TTGCTGCACATCGAGTGTCT |
| Â | Rev-TGCCGAAGAAAACGCTGTTG |