Temporal and dynamic changes in chromatin states. (A) Numbers of significant chromatin state changes (all clusters confounded) at the D0/D3 and D3/D9 transitions (t-test with Bonferroni correction). (B) Dynamics of chromatin states in each expression cluster. Graphs represent the sum of changes in P-values for each cluster. (C) Matrix of significant differences in chromatin state dynamics between clusters: clusters in columns (top) show greater state dynamics than clusters in rows (left). P-values for (B) and (C) are shown in Additional file 2: Table S6B (one-sided Wilcoxon tests). (D) Browser view of chromatin state changes and RNA-seq profiles at the PPARG locus during differentiation; the red boxed area is enlarged (zoom, right).