TF binding preferences |
---|
TF function | Unmethylated DNA | Methylated DNA | Both |
Activator | (1) negative SCC
M/E
| (2) positive SCC
M/E
| insignificant SCC
M/E
|
Repressor | (3) positive SCC
M/E
| (4) negative SCC
M/E
| insignificant SCC
M/E
|
Both | insignificant SCC
M/E
| insignificant SCC
M/E
| |
- There are four possible scenarios of interaction of DNA methylation and TF functions:
- (1) TF can bind unmethylated DNA and cannot bind methylated DNA. TF acts as a transcription activator. The methylation profile of cytosines within TFBS should be negatively correlated with TSS expression.
- (2) TF can bind methylated DNA and cannot bind unmethylated DNA. TF acts as a transcription activator. The methylation profile of cytosines within TFBS should be positively correlated with TSS expression.
- (3) TF can bind unmethylated DNA and cannot bind methylated DNA. TF acts as a transcription repressor. The methylation profile of cytosines within TFBS should be positively correlated with TSS expression.
- (4) TF can bind methylated DNA and cannot bind unmethylated DNA. TF acts as transcription repressor. The methylation profile of cytosines within TFBS should be negatively correlated with TSS expression.