Figure 4

DNA methylation levels around the TSS, gene body, and TTS across five gene expression level. The DNA methylation profile in the gene region was shown by the reads that were aligned on a unique locus in the genome. The gene region referred to those regions in the 2 kb region upstream of the TSS, the gene body from TSS to TTS, and the 2 kb region downstream of the TTS. In upstream and downstream 2 kb regions, the regions were split into 20 non-overlapping windows, and the average alignment depth was calculated for each window. In the gene body, each gene was split into 40 equal windows, and the average alignment depth was calculated for each window. The Y-axis represents the average of the normalized depth for each window. Genes were divided into five groups according to expression levels: 0 (silent genes), 0–1 (low–level expressed genes), 1–20 (middle–level expressed genes), 20–1000 (high–level expressed genes), and > 1000 (highest–level expressed genes, house-keeping genes). The DNA methylation profile in and around gene bodies were compared across these five gene expression levels. (A): SCNT placenta; (B): control placenta.