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Table 3 Pigment content in leaves

From: Transcriptomic and proteomic analyses of a pale-green durum wheat mutant shows variations in photosystem components and metabolic deficiencies under drought stress

Pigment

Control

Water stress

 

WT

M

M vs WT (%)

P-value

WT

M

M vs WT (%)

P-value

Chlorophyll a

16308

9704

-40

0.0315

7607

6804

-11

n.s.

Chlorophyll b

4700

2374

-49

0.0106

1901

1609

-15

n.s.

Total chlorophylls

21007

12078

-43

0.0247

9508

8413

-12

n.s.

Ratio a/b

3.46

4.08

18

0.0001

4.03

4.24

5

n.s.

Violaxanthin (V)

968

614

-37

0.0293

535

453

-15

n.s.

Neoxanthin

296

171

-42

0.0095

136

97

-28

n.s.

Antheraxanthin (A)

147

90

-38

n.s.

86

39

-54

0.0152

Lutein

1745

856

-51

0.0071

834

649

-22

n.s.

Zeaxanthin (Z)

253

258

2

n.s.

224

214

-5

n.s.

β-Carotene

1205

734

-39

0.0464

598

566

-5

n.s.

Total carotenoids

4614

2723

-41

0.0162

2357

2019

-14

n.s.

(0.5A + Z)/(VAZ)

0.24

0.32

33

0.0096

0.34

0.33

-3

n.s.

  1. Pigment quantification (μg/g dw) of the flag leaf of wild-type (WT) and pale-green mutant (M) plants at anthesis under control and water stress conditions was performed by HPLC. Values are means of four samples. Xanthophylls de-epoxidation rate [(0.5A + Z)/(VAZ)].