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Figure 1 | BMC Genomics

Figure 1

From: The CtrA phosphorelay integrates differentiation and communication in the marine alphaproteobacterium Dinoroseobacter shibae

Figure 1

Phenotypic characterization. (A) Growth curves of D. shibae DFL12 wild-type and mutant strains cultured in minimal see water medium supplemented with 5 mM succinate at 30°C. The y-axis is drawn in ln scale. (B) Phase contrast microscopy of corresponding strains. Scale bar represents 20 μm. (C) Flow cytometric representation of morphological differences between mutant and wild-type strains based on size (FSC, forward scatter) and granularity (SSC, side scatter). Numbers in plots (top right quadrant) indicate percent larger cells in this area. (D) The chromosome equivalent profiles of the ∆ctrA (red line), (E) ∆cckA (green line) and (F) ∆chpT mutant (blue line) from cultures used in (A) were compared to wild-type (grey area) and ∆luxI 1  (black line) cultures by flow cytometry of SYBR Green I-stained cells (50,000 events counted). The x and y axes are in log2 scale and represent fluorescence intensity and cell density, respectively. The analysis presented in B-F was carried out with cultures in the mid-exponential phase.

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