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Figure 5 | BMC Genomics

Figure 5

From: The CtrA phosphorelay integrates differentiation and communication in the marine alphaproteobacterium Dinoroseobacter shibae

Figure 5

Regulation of cell cycle related genes and QS genes. (A) Heat map showing gene expression of cell cycle related genes and (B) QS genes. The autoinducer binding domain gene Dshi_4067 has been named luxB. Red stars indicate putative CtrA binding sites found at the promoters of these genes. Color bar as in Figure 3. (C) The promoter region of the LuxR2I2 operon showing putative CtrA binding sites. (D) Production of long-chain AHLs of D. shibae wild-type, knock-out mutant strains and their respective genetic complementations ∆ctrA pHW1 (short: pHW1), ∆cckA pHW1 (short: pHW2) and ∆chpT pHW1 (short: pHW3). Results are presented as fold induction obtained by dividing the specific fluorescence intensity of the samples by that of the negative control (DCM). Values represent means and standard deviations of three biological replicates. Asterisks indicate statistically significant differences between complementation strain and respective knock-out mutant calculated using Student’s t test (***p < 0.001).

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