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Table 1 Identification of proteins in royal jelly

From: Comprehensive identification of novel proteins and N-glycosylation sites in royal jelly

Classification

Accession

-10lgP

Coverage (%)

Matches

Unique

Mass(Da)

Protein name

SignalP

PSORT

YELLOW/MRJP family

gi|58585098

566.72

96

742

166

48886

Major royal jelly protein 1 precursor

-

-

gi|58585108

527.66

93

437

100

51074

Major royal jelly protein 2 precursor

-

-

gi|58585142

487.03

88

354

12

61662

Major royal jelly protein 3 precursor

-

-

gi|284182838

357.38

89

236

15

53015

Major royal jelly protein 4

-

-

gi|284812514

386.92

71

182

6

70182

Major royal jelly protein 5

-

-

gi|58585188

282.69

58

57

7

49786

Major royal jelly protein 6 precursor

-

-

gi|62198227

422.37

84

182

70

50541

Major royal jelly protein 7 precursor

-

-

gi|58585070

131.13

15

3

3

46956

Major royal jelly protein 8 precursor

-

-

gi|67010041

221.7

45

26

10

48688

Major royal jelly protein 9 precursor

-

-

gi|148277624

146.46

19

6

6

48235

Yellow-e3 precursor*

#

Metabolic activity

gi|328787887

299.74

26

40

37

188194

Lysosomal alpha-mannosidase

-

-

gi|89885579

290.12

50

33

3

65565

Alpha-glucosidase

-

-

gi|58585144

227.31

43

7

3

55947

Alpha-amylase precursor

-

-

gi|66564326

185.29

37

10

6

52947

Plasma glutamate carboxypeptidase isoform 1

-

-

gi|328778095

116.42

10

4

4

56432

Lysosomal Pro-X carboxypeptidase*

#

gi|66560290

102.39

10

3

3

42222

Lysosomal aspartic protease*

#

gi|328782027

93.88

6

4

4

88720

Membrane metallo-endopeptidase 1*

#

gi|48118838

276.66

56

32

31

58571

Glucosylceramidase

-

-

gi|66524161

196.85

59

15

14

25186

Ferritin heavy chain

-

-

gi|328779534

220.38

10

23

20

79344

Hypothetical protein LOC552041

-

-

gi|328780642

167.48

16

9

8

64654

Matrix metalloproteinase 14*

#

gi|328784061

154.24

27

6

5

34132

Pancreatic triacylglycerol lipase*

#

Health promotion activity

gi|58585090

389.52

76

137

77

67938

Glucose oxidase

-

-

gi|166795901

259.92

93

46

14

21348

Apolipophorin-III protein precursor

-

-

gi|328782084

240.41

41

7

4

59502

Antithrombin-III

-

-

gi|60115688

226.26

71

39

5

24819

Icarapin precursor

-

-

gi|254910938

214.17

75

14

4

10717

Defensin-1 preproprotein

-

-

gi|187281543

150.07

16

11

10

87937

Venom dipeptidyl Peptidase 4 precursor*

#

gi|110755367

142.44

13

7

7

75706

Toll-like receptor 13 isoform 1

-

-

gi|48101366

138.63

15

5

5

44639

Venom serine protease 34*

#

gi|254548157

102.39

37

3

2

12611

Hymenoptaecin precursor*

#

gi|328790726

243.78

70

28

19

42665

Venom acid phosphatase Acph 1

-

-

gi|66507455

188.31

27

11

11

39483

Venom protease*

#

gi|328792524

89.27

9

5

2

90763

Hypothetical protein LOC408570*

#

gi|110758964

221.47

83

17

4

10161

Regucalcin

-

-

gi|66565246

83.06

16

3

2

17081

Lysozyme isoform 1*

#

Developmental process

gi|66514614

165.33

19

8

4

48741

Idgf4

-

-

gi|110766389

139.29

22

5

5

30201

Protein takeout

-

-

gi|66521538

101.17

11

2

2

33735

Protein CREG1*

#

gi|94158822

86.71

22

2

2

15201

Odorant binding protein 14 precursor

-

-

Unknown

gi|48094573

292.99

64

45

32

19434

Hypothetical protein LOC408608

-

-

 

gi|110763647

109.47

27

5

4

18478

Hypothetical protein LOC726323

-

-

  1. Note: All of the identified proteins are of Apis mellifera origin. Accession is the unique number given to mark the entry of a protein in the database of Apis (downloaded April 2012, version 4.5 of the honeybee genome) using in-house PEAKS software (version 6.0, Bioinformatics Solutions Inc.). “-10logP” is the score calculated by PEAKS software. Sequence coverage is the ratio of the number of amino acids in every peptide that matches with the mass spectrum divided by the total number of amino acids in the protein sequence. Matches are the number of experiment fragmentation spectra paired to a theoretical segment of protein. The number of unique peptides refers to the peptide sequences that are unique to an individual parent protein sequence. SignalP refers to the result researched with SignalP 4.1. PSORT refers to the result researched with PSORT II. “*” indicates the protein identified as novel in royal jelly. “√” indicates the protein identified with signal peptide by SignalP 4.1. “#” indicates the protein identified as extracellular by PSORT II. “-” indicates the protein did not be researched with SignalP 4.1 or PSORT II.